Question

The recognition site of some restriction enzymes are shown. Which will produce sticky ends? EcoRI    G↓AATTC SmaI      CCC↓GGG...

The recognition site of some restriction enzymes are shown. Which will produce sticky ends?

EcoRI    G↓AATTC

SmaI      CCC↓GGG

XhoI      C↓TCGAG

a. EcoRI and SmaI only

b. EcoRI and XhoI only

c. SmaI and XhoI only

d. SmaI only

e. XhoI only

f.All the enzymes shown will leave sticky ends

g. EcoRI only

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Answer- (b) EcoRl and Xhol only will produce stictly end. Sticky end generated by type ll restriction endonuclease. Will is very easy to ligate that's why used in recombinant DNA technology. Please go through the image once:

For further queries please do comment. Thank you ?.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Which statement regarding recombinant DNA is false? Select one: a. Restriction enzymes cut DNA at palindromic...
Which statement regarding recombinant DNA is false? Select one: a. Restriction enzymes cut DNA at palindromic sequences. b. A single restriction enzyme can cut DNA sequences at several different recognition sites. c. Restriction enzymes can create DNA fragments with “blunt ends.” d. Complementary “sticky ends” can form hydrogen bonds with each other. e. Restriction enzymes can create DNA fragments with “sticky ends.”
Restriction enzymes, also known as restriction endonucleases, are enzymes that cut a DNA molecule at a...
Restriction enzymes, also known as restriction endonucleases, are enzymes that cut a DNA molecule at a particular place. They are essential tools for recombinant DNA technology. The enzyme "scans" a DNA molecule, looking for a particular sequence, usually of four to six nucleotides. Once it finds this recognition sequence, it stops and cuts the strands. This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the...
Ligation reactions occur most efficiently between DNA fragments: A. that have sticky ends. B.   that have...
Ligation reactions occur most efficiently between DNA fragments: A. that have sticky ends. B.   that have blunt ends. C. that have complementary sticky ends. D. that are cleaved with different restriction enzymes. E. where one fragment has a sticky end and the other has a blunt end.
please answer all 7-11 7. Which is not true for enzymes: A. The active site is...
please answer all 7-11 7. Which is not true for enzymes: A. The active site is a three dimensional crevice in the protein. B. Enzymes produce a more negative value for ΔG. C. Most substrates bind enzymes by forming multiple weak interactions. D. The active site takes up only a small part of the enzyme’s volume. 8. What are the two types of cofactors associated with enzymes? (6 points) 9. Explain the function of the following substances in solid phase...
Examine the following restriction recognition sequence. a). (One point)Your mission is to complete the entire recognition...
Examine the following restriction recognition sequence. a). (One point)Your mission is to complete the entire recognition sequence, which is palindromic. 5’-G/T C _ _ _-3’ 3’-_ _ _ _ _ _-5’ b). (3 points) The dashed line indicates the endonuclease cut site. Draw out the result after digestion with the enzyme, labeling all 5’ and 3’ ends. T his enzyme leaves (CIRCLE the correct answer): 3’ overhangs or 5’-overhangs
A circular plasmid of 6.0 kB was cleaved with the restriction enzymes EcoRI, BamHI and PstI.  Cleavage...
A circular plasmid of 6.0 kB was cleaved with the restriction enzymes EcoRI, BamHI and PstI.  Cleavage with each enzyme alone gave a single band of 6.0 kB for BamHI and PstI, and a single band of 3.0 kB for EcoRI.  When BamHI and PstI were used together for the digest, bands of 1.5 and 4.5 were observed, and when BamHI and EcoRI were used together only the 3.0 kB band was observed.  What do you predict will be the pattern generated by...
a) Enzymes perform the role of _________ (b) Some enzymes require another molecule (either organic or...
a) Enzymes perform the role of _________ (b) Some enzymes require another molecule (either organic or non-organic) to be present in order for the reaction to take place. These molecules are called what? (c) Enzymes that are able to act on a wide range of substrates, but are specific for a single type of reaction, can be explained by which model of enzyme/substrate interaction? (d) What is the classification of enzymes that cleave bonds with water? (e) Which group of...
Which of the following involves the insertion of a chemically synthesized DNA containing an insertion/deletion of...
Which of the following involves the insertion of a chemically synthesized DNA containing an insertion/deletion of a nucleotide in the sequence to create specific genetic change? A. Combinatorial biosynthesis B. Site-directed mutagenesis C. Metagenomics D. Protoplast fusion Which of the following is true about restriction enzymes? A. Recognizes non-specific sites of DNA B. Used to promote viral infections C. Can cut a DNA fragment to create “sticky” ends that are utilized for generating recombinant DNA D. A and C
Which of the following techniques makes use of reverse transcriptase? Group of answer choices Homologous recombination...
Which of the following techniques makes use of reverse transcriptase? Group of answer choices Homologous recombination Northern blotting PCR Automated Sanger sequencing All of the above None of the above. ------------------------------- Restriction enzymes: Group of answer choices A.) Help protect bacteria from foreign DNA.e B) Always generate “sticky ends" with either 3' or 5' overhangs C) Will not cut at a recognition site if the DNA is methylated. D) Both A and C. All of the above. ------------------------------------------------- DNA polymerase...
Restriction enzymes recognize palindrome sequences, which are also often involved in gene regulation. What is the...
Restriction enzymes recognize palindrome sequences, which are also often involved in gene regulation. What is the last half of the GGA palindrome sequence and HOW do we figure out this? a) GGA b) AGG c) TCC d) CCT
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT