Question

Examine the following restriction recognition sequence.

a). (One point)Your mission is to complete the entire recognition sequence, which is palindromic. 5’-G/T C _ _ _-3’ 3’-_ _ _ _ _ _-5’

b). (3 points) The dashed line indicates the endonuclease cut site. Draw out the result after digestion with the enzyme, labeling all 5’ and 3’ ends. T

his enzyme leaves (CIRCLE the correct answer): 3’ overhangs or 5’-overhangs

Answer 1

a. Palindromic sequence is the sequence which reads same on both the strands.

Eg: 5' - GAATTC - 3'

3' - CTTAAG - 5'

For the given incomplete sequence:

5' - GTCGAC - 3'

3' - CAGCTG - 5'

b. The restriction enzyme cuts this site by cutting in between G and T from the 5' end. Hence after restriction, the cut strands overhangs will be as follows:

5' - G -3'    5' - TCGAC - 3'

3' - CAGCT - 5'    3'-G - 5'

Hence it produces both the 3' and 5' overhangs.

The 5' overhang is TCGAC and 3' overhang is G.

If you have any query kindly comment before giving thumbs up. Thank you.