Question

Which of the following techniques makes use of reverse transcriptase?

Group of answer choices

Homologous recombination

Northern blotting

PCR

Automated Sanger sequencing

All of the above

None of the above.

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Restriction enzymes:

Group of answer choices

A.) Help protect bacteria from foreign DNA.e

B) Always generate “sticky ends" with either 3' or 5' overhangs

C) Will not cut at a recognition site if the DNA is methylated.

D) Both A and C.

All of the above.

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DNA polymerase catalyzes leading strand synthesis of DNA in a _____ direction and lagging strand synthesis of DNA in a _____ direction.

Group of answer choices

5'-->3’, 5’-->3’

5'-->3’, 3’-->5’

3'-->5’, 5’-->3’

3'-->5’, 3’-->5’

All of the above

Answer 1

Answer 1-a) Homologous recombination

Answer 2-e) all of the above

All the statements are correct as :-

bacteria uses a restriction enzyme to defend against bacteriophages, or phages.

Restriction endonuclease uses 3' or 5' overhangs also called sticky ends to form base pairs with DNA molecules.

Answer 3- a) 5'-->3’, 5’-->3’

The leading as well as the lagging strand is synthesized in 5'-3' direction but the leading strand is synthesized continuously where as lagging strand synthesized in short fragments.