Question

Explain how to:

-Make 1/100 dilution of the yeast suspension using normal saline as diluent.

-Mix equal volumes of yeast and Trypan blue

-Load the hemocytometer. If your sample is too dense, dilute your sample some more

Answer 1

Answer:- Its easy. First you take 1 ml of the yeast suspension that is provided to you and add 99 ml of normal saline to it. This way you will get 1/100 dilution of yeast suspension.

Now mix 5 ?L (?L = microliter) of 1/100 yeast suspension and 5 ?L of trypan blue dye. Make sure the total volume of the mixture is not more than 10 ?L, as it will be difficult to load more amount in the heamocytometer properly.

Now load the mixture on to the heamocytometer. Count the yeast cells. If they are more than 100 cells in one quadrant then it means the sample is too dense. You can then dilute the sample more accordingly as described above.