Question

Please go step by step! I really want to understand, thank you!

7) You are given a culture of cells. You mixed 10uL of the diluted cell culture with 10uL of trypan blue and loaded a hemocytometer. You counted a total of 105 cells in all four quadrants. Answer the following questions below and SHOW OR EXPLAIN YOUR WORK.

A. How many cells/mL does the original cell culture contain?

B. You are asked to make a 0.5mL of working stock cell suspension that contain 500 cells/mL. How would you make this working stock cell suspension? Use the equation C1 x V1 = C2 x V2 to solve this problem. Be sure to note what C1, V1, C2 and V2 is. Write your final answer in microliters (uL).

C. One microliter is the smallest amount you can pipette accurately. How would you serially dilute your original cell sample so you can accurately create a 0.5mL working stock suspension of 500 cells/mL. Be sure to redo all your calculations and explain in the end how you would mix your cells and media to create your working stock. Keep your final answers to one decimal point (such as 1.1uL or 2.7uL). Be sure to include how much volume of media you would add to your diluted cells in your final answer.

Answer 1