Question

Please go step by step! I really want to understand, thank you! 7) You are given...

Please go step by step! I really want to understand, thank you!

7) You are given a culture of cells. You mixed 10uL of the diluted cell culture with 10uL of trypan blue and loaded a hemocytometer. You counted a total of 105 cells in all four quadrants. Answer the following questions below and SHOW OR EXPLAIN YOUR WORK.

A. How many cells/mL does the original cell culture contain?

B. You are asked to make a 0.5mL of working stock cell suspension that contain 500 cells/mL. How would you make this working stock cell suspension? Use the equation C1 x V1 = C2 x V2 to solve this problem. Be sure to note what C1, V1, C2 and V2 is. Write your final answer in microliters (uL).

C. One microliter is the smallest amount you can pipette accurately. How would you serially dilute your original cell sample so you can accurately create a 0.5mL working stock suspension of 500 cells/mL. Be sure to redo all your calculations and explain in the end how you would mix your cells and media to create your working stock. Keep your final answers to one decimal point (such as 1.1uL or 2.7uL). Be sure to include how much volume of media you would add to your diluted cells in your final answer.

Homework Answers

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Use the information below to answer the following questions. After taking an aliquot of J558 suspension...
Use the information below to answer the following questions. After taking an aliquot of J558 suspension cells from your T75 culture flask, you dilute this aliquot in Trypan Blue in a 1:1 ratio. After loading the cells on the hemocytometer you obtain the following counts: Transparent cells Blue cells Quadrant 1 32 1 Quadrant 2 31 1 Quadrant 3 30 1 Quadrant 4 31 2 Quadrant 5 33 1 1) What is the cell viability? 2) What is the density...
E. coli culture is given. Turbidity assay 1 OD 600 (optical density measured at 600 nm)...
E. coli culture is given. Turbidity assay 1 OD 600 (optical density measured at 600 nm) of this culture correlates with 1 x 10^8 cells per mL. Q-1. how would I make 1 mL of a diluted culture of this stock that would give a 0.25 OD 600 . (fill in the blanks below) I would add __________ (µL) of the original culture to __________ (µL) of media to obtain a _____ times diluted 1 mL media that would give...
7. 25 ml of 6 M NaOH was diluted with water to 400 ml. What is...
7. 25 ml of 6 M NaOH was diluted with water to 400 ml. What is the pH and pOH of the final solution? What is the [H+] and [OH-]? (Remain calm; remember the formula for dilution from the previous lab session: (V1)(C1) = (V2)(C2) ) 8. A solution was made by dissolving 10 g of NaOH (formula weight 40 g/mole) in water and the final volume brought to 500 ml. What is the pH, pOH, [H+] and [OH–] of...
To subculture cells, you must plate the cells on a new plates at a specific density...
To subculture cells, you must plate the cells on a new plates at a specific density that depends on that cell line and the purpose of your experiment. If you needed to plate the cells(ie. That your group resuspended in 50microliters) at a concentration of 2200 cells per cm2 onto 35mm plates that have an effective growth surface area of 9cm2, how would you do this? The final volume of media for a 35mm plate is 2mL. A.Total # of...
1. Suppose you were to separate 5 ml of blood and have a 65% recovery of...
1. Suppose you were to separate 5 ml of blood and have a 65% recovery of the white blood cells. Suppose further that 80% of those cells were viable. You resuspend the recovered cells in 10 ml of media (RPMI + FCS, keeps the cells from blowing up). You then dilute them 1:10 in the same medium, and follow the protocol for Trypan blue staining. Based on what you know of the percentage of white blood cells in blood, how...
I have done these problems but am unsure if the answers I got were correct, Please...
I have done these problems but am unsure if the answers I got were correct, Please show all steps so I can fully understand! 1.) How many microliters of original sample are required to produce a final dilution of 10-3 in a total volume of 1 mL?       INFO: 1 microliter is 10-6 L or 10-3 mL. 2.) If you suspect your culture of bacteria has 240 x 106 cells per mL, what would you want the final dilution to be...
I have a tricky dilution question. You have a stock solution of methylene blue at 1g/100ml....
I have a tricky dilution question. You have a stock solution of methylene blue at 1g/100ml. Make 250ul of methylene blue at a concentration of 150ug/100ml with as little waste as possible (have as little excess as possible). So I have the dilution formula C1V1 = C2V2. That means (1g/100ml)V1 = (150ug/100ml)(250ul). I converted C1 to ug and got 106ug/100ml which reduces down to 104ug/1ml. Please correct anything I may have wrong. Next I cnverted V2 to ml to keep...
You are a directed studies student (undergraduate student conducting research in a lab for course credit)...
You are a directed studies student (undergraduate student conducting research in a lab for course credit) and your supervisor asks you to use a cryopreserved cell culture stock to determine where an enzyme is located in the cell and to measure its activity.   The cryopreserved yeast or E. coli cells have been genetically modified to express a synthetic enzyme. During the synthesis of this enzyme it is thought that the enzyme could be incorporated into different membranes within the cell....
2. RDRPkill has a molecular weight of 400. You have a small, concentrated 500 mM stock...
2. RDRPkill has a molecular weight of 400. You have a small, concentrated 500 mM stock solution. a. Express this concentration in the following units: i. M ii. μg/μL iii. % (w/v) iv. weight/dL b. How much of this compound would you have to weigh out if you were to make up 500 µL of the solution? c. If you added 5 μL of a 500 mM solution to a cell incubation with a total volume of 2 mL i....
Part III – Something's Not Right “It’s good to have you home, honey. I missed you....
Part III – Something's Not Right “It’s good to have you home, honey. I missed you. How was the flight?” Stacey had come to the airport to pick Frank up and she leaned over to kiss him as he climbed into the car with his luggage. “How were the meetings? You look tired,” she added. “The past week was intense and I am exhausted. I thought I would manage some R & R during the trip, but no such luck....
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT