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Which of the following methods used to monitor RNA concentrations in a sample would be best...

Which of the following methods used to monitor RNA concentrations in a sample would be best to follow the expression levels of all genes in human tissue?

quantitative PCR (qPCR)

RNA Sequencing

Microarray

Northern Blot
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Answer: RNA sequencing and Microarray are the methods used to determine the expression levels of all genes in a human tissue. In RNA sequencing, all the mRNA of the tissue that represents all the expressed genes are sequenced and the sequence data is used to generate expression levels on the genes. In Microarray, small DNA probes that represents all the genes of human tissue is spotted on the solid chip. The mRNA sample of the human tissue is hybridized to the chip and the hybridization signal is used to determine the expression levels of all the genes.

Quantitative PCR and Northern blotting are used to quantify the expression levels of few genes but not all the genes in the human tissue. This is because these methods are low throughout techniques.

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