Question

Which of the following methods used to monitor RNA concentrations in a sample would be best...

Which of the following methods used to monitor RNA concentrations in a sample would be best to follow the expression levels of all genes in human tissue?

quantitative PCR (qPCR)

RNA Sequencing

Microarray

Northern Blot
Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Answer: RNA sequencing and Microarray are the methods used to determine the expression levels of all genes in a human tissue. In RNA sequencing, all the mRNA of the tissue that represents all the expressed genes are sequenced and the sequence data is used to generate expression levels on the genes. In Microarray, small DNA probes that represents all the genes of human tissue is spotted on the solid chip. The mRNA sample of the human tissue is hybridized to the chip and the hybridization signal is used to determine the expression levels of all the genes.

Quantitative PCR and Northern blotting are used to quantify the expression levels of few genes but not all the genes in the human tissue. This is because these methods are low throughout techniques.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Which of the following would be best suited to measure the amount of insulin mRNA produced...
Which of the following would be best suited to measure the amount of insulin mRNA produced in a pancreatic cell line? A. Northern Blot B. Southern Blot C. Sanger sequencing D. Any of these would work
1.A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection...
1.A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection process is the _____ blot. Select one: a. Northern b. Western c. Northeastern d. Southern e. Southwestern 2. DNA sequencing by controlled termination of replication is called the _____ method. Select one: a. Sanger b. E. coli c. Restriction enzymes d. DNA Microarray e. Ligase f. Polymerase Chain Reaction g. Footprint h. Reverse Trancriptase i. Vector j. Expression k. Fluorescent l. cDNA 3. How...
Q1. Which of the following is a sequencing-based technique that can be used to compare the...
Q1. Which of the following is a sequencing-based technique that can be used to compare the change in expression of all of the genes in treated and untreated cells to determine how gene expression changes due to the treatment? a) GWAS b) Gene expression microarray c) RNAseq d) Metagenomics Q2. Which of the following statements is CORRECT? a) The most common site of DNA methylation is the guanine CpG islands. b) Vinclozolin was found to only affect the mice exposed...
Sam68 protein •Member of STAR family of RNA binding proteins •STAR= signal transduction and activation of...
Sam68 protein •Member of STAR family of RNA binding proteins •STAR= signal transduction and activation of RNA •STAR proteins regulate mRNA stability, export, splicing and translation Mice were bred for 5 mo with wild-type females of proven fertility and # of litters and pups tallied. Pronuclei (indicated by arrowheads in B) in the fertilized eggs were used to score fertilization. A graph showing the results of three independent mating experiments is shown in C. (B) Quantitative real-time PCR analysis of...
To determine whether regulation of gene expression by short RNAs was a naturally occurring phenomenon, researchers...
To determine whether regulation of gene expression by short RNAs was a naturally occurring phenomenon, researchers isolated RNA from a cell and fractionated them by size to obtain only short RNAs. The next step was to clone these molecules. Today the cloning step would not be required. Which of the techniques below is the best reason why? Because a microarray could tell if the fragments were encoded by the genome. Because a PCR reaction could tell if the fragments were...
Which of the following statements best describes the process that characterizes inferential statistics? Descriptive methods are...
Which of the following statements best describes the process that characterizes inferential statistics? Descriptive methods are used to characterize the information in a population so that conclusions may be reached regarding samples taken from that population. Descriptive methods are used to obtain parameters that are then used to make inferences about the sample. Descriptive methods are used to obtain statistics that are then used to make inferences about the population. Finite populations are aggregated to obtain (nearly) infinite populations that...
Procedure I. Reverse Transcription. For each of your RNA samples set up the following reaction: *Remember...
Procedure I. Reverse Transcription. For each of your RNA samples set up the following reaction: *Remember to use proper RNA handling technique! 1. In a 0.2 ml size RNAse-Free tube add the following: • 0.5 µg RNA (no more than 6 µl) You can add as little as 0.1 µg of RNA if your concentration is low • 2 µl dT primer • Nuclease Free Water to final volume of 8 µl * Before you start write the exact volume...
Which of the following would work best in Western blot if you could not detect low...
Which of the following would work best in Western blot if you could not detect low amounts of protein in the SDS-PAGE? 1. Use HRP-Ni2+ conjugate for the bovine LDH 2. Use a primary antibody for barracuda and the anti-LDH human secondary antibody 3. Use a primary antibody with a Nickel binding site to recognize a primary antibody to barracuda DNA 4. Use HRP-Ni2+ conjugate with a His tagged LDH 5. We would not be able to detect protein in...
which of the following time series forecasting methods would not be used to forecast seasonal data?...
which of the following time series forecasting methods would not be used to forecast seasonal data? dummy variable regression simple exponential smoothing time series decomposition multiplicative Winters method
Which ONE of the following is NOT TRUE about noncoding RNAs: microRNAs (~22 nucleotides long) regulate...
Which ONE of the following is NOT TRUE about noncoding RNAs: microRNAs (~22 nucleotides long) regulate gene expression via RNA degradation microRNAs regulate genes expression via inhibition of translation microRNAs are encoded as genes in the human genome and can act as either oncogenes or tumor suppressor genes long non coding RNAs (lncRNAs) are 20,000+ nucleotides long QUESTION 2 Which ONE of the following is NOT a method by which a tumor suppressor gene can lead to cancer: incorporation into...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT