Question

Q1. Which of the following is a sequencing-based technique that can be used to compare the...

Q1. Which of the following is a sequencing-based technique that can be used to compare the change in expression of all of the genes in treated and untreated cells to determine how gene expression changes due to the treatment?

a) GWAS

b) Gene expression microarray

c) RNAseq

d) Metagenomics

Q2. Which of the following statements is CORRECT?

a) The most common site of DNA methylation is the guanine CpG islands.

b) Vinclozolin was found to only affect the mice exposed to it. Their offspring were normal.

c) X-chromosome inactivation can result in individuals who exhibit different traits at different locations on or in their body.

d) Chemical modification of histone proteins is not a molecular mechanism that alters chromatin structure.

Q3. Which of the following best characterizes many cancers such as colon cancer?

a) They result when the transition from G2 to M in the cell cycle is inhibited.

b) They result when DNA replication during the S period of the cell cycle is inhibited.

c) They result from the activation of one critical tumor-suppressor gene.

d) They result from a series of sequential mutations in a number of genes.

e) They result from decreased expression in a series of cellular oncogenes.

Homework Answers

Answer #1

Ans. 1. Option (C) RNAseq. Differential gene expression can be determined by detecting transcriptome (total mRNA) of the cells before and after the treatment. Transcriptome (total mRNA) can be detected by RNA next generation sequencing (RNAseq).

A genome-wide association study (GWAS) is an approach used in genetics research to find specific genetic variations/ marker associated with particular diseases to detect the diseases.

Gene expression microarray is the DNA/ probe- mRNA (target gene expressed) hybridization based techniques to detect gene expression.

Metagenomics is the study of genetic material recovered directly from environmental samples/ without culturing the microorganism..

Note: Please rest questions separately as I'm supposed to answer only one question in given time.

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