Question

How does RNA polymerase bind DNA? which subunit binds first? Provide the sequence of a bacterial...

How does RNA polymerase bind DNA? which subunit binds first?

Provide the sequence of a bacterial promoter sequence

What are the differences between TRP operon and Lac operon?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

The alpha subunit bind to very upstream sequence of the DNA and then sigma helps in binding with promoter.

RNA polymerase has many subunits like 2 alpha one beta one beta ' one omega which form core enzyme and one sigma which is coenzyme ।when sigma binds the core ENZYME it becomes holoenzyme.

This alpha subunits bind upstream of the promoter and then, sigma subunit binds to the promoter sequences by hydrogen bonding and melt the DNA and start the transcription.

If has to concensus sequences in -10 and. -35 sequences .

In - 10 position, there is TATAAT AND - 35 POSITION , TTGACA.

TRYP AND LAC OPERON IS OPPOSITE IN SENSE THAT LAC OPERON IS INDUCIBLE OPERON WHICH MEANS WHEN LACTOSE IS PRESENT LACTOSE METABOLIZING ENZYME OPERON WILL BE TRANSCRIBED AND WILL METABOLIZE LACTOSE.

BUT IN TRP OPERON WHEN TRYPTOPHAN IS PRESENT , THEN TRYPTOPHAN PRODUCING ENZYME WILL NOT BE PRODUCED.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
1. As the RNA polymerase is synthesizing new RNA, it uses one strand of the DNA...
1. As the RNA polymerase is synthesizing new RNA, it uses one strand of the DNA as a template and it adds a new nucleotide to which end of the growing RNA strand? As it moves along the DNA template, which direction is the polymerase moving? 2. In eukaryotes, the genomic DNA is packaged with proteins called histones. What is the term for the combination of DNA + histones? 3. In eukaryotes, when the initial RNA is transcribed, is it...
Many eukaryotic genes have regions called enhances. What is an enhancer? a. RNA polymerase binds here...
Many eukaryotic genes have regions called enhances. What is an enhancer? a. RNA polymerase binds here to begin transcription. b. a DNA sequence close to the promoter where a specific protein can bing to activate translationc. c. DNA seqeuence far from the promotoer where a specific protein can bind to activate transcription. d. ribosomes bind here to begin translation e. snRNP's bind here
1. To initiate transcription, RNA polymerase must recognize the ____ sequence on DNA. 2. Ribosomal 30S...
1. To initiate transcription, RNA polymerase must recognize the ____ sequence on DNA. 2. Ribosomal 30S subunit binds to mRNA by recognizing ______ sequence upstream of the start codon. (Full name for full credit) 3. A special initiator tRNA called ______ binds to the ______ site of the 70S ribosome for base pairing with the ATG start codon. 4. If RNA polymerase transcribes a long mRNA encoding more than one polypeptide, it is called a (an) _______ RNA. 5. Some...
The famous -10 and -35 DNA sigma factor binding motifs function as? A. DNA sequence landmarks...
The famous -10 and -35 DNA sigma factor binding motifs function as? A. DNA sequence landmarks that ensure proper sigma favor binding to promoter regions, and as a result bring along RNA polymerase to park at a position enabling transcription at position +1. B. the -10 motif binds inducers (e.g., Cap/cAMP) and -35 motif binds a repressor (e.g., LacI). C. DNA sequence landmarks that interact directly with RNA polymerase placing the transcription initiation at position +1. D. transcription repressors, preventing...
At which site on the DNA of a gene does RNA polymerase release its newly made...
At which site on the DNA of a gene does RNA polymerase release its newly made RNA? Choose one: A. poly-A tail B. stop codon C. promoter D. TATA box E. terminator
Please provide brief (one sentence) answers: a) The Mycoplasma chromosome codes for 500 genes, yet it...
Please provide brief (one sentence) answers: a) The Mycoplasma chromosome codes for 500 genes, yet it has about 20 promoters. Provide a brief explanation for how this is possible. b) How many seconds would it take the bacterial RNA polymerase to synthesize an mRNA of 5,000 bases? c) How many mistakes would the bacterial RNA polymerase make while synthesizing an mRNA of 30,000 bases? d) Sigma (?) protein by itself does not bind to promoter sites. Predict the effect on...
1. In the lac operon RNA polymerase first binds to the: a. promoter b. operator c....
1. In the lac operon RNA polymerase first binds to the: a. promoter b. operator c. repressor d. regulator 2. Spontaneous mutations are: a. cause by chemicals such as acridine and caffeine b. caused by physical agents such as ultraviolet light or x-rays c. the result of errors in the base pairing of nucleotides during replication d. occur at a rate higher than the rate of induced mutations 3. Which statement about mutations is false? a. A mutation can alter...
Put the following statements in the order in which they occur assuming transcription happens first. A:...
Put the following statements in the order in which they occur assuming transcription happens first. A: The initiator tRNA is located in the p-site of the ribosome B: 50S large subunit of ribosome associates with the m-RNA-30S initiator tRNA complex C: Initiator tRNA IF-2 GTP complex associates with pre-initiator complex D: RNA polymerase holoenzyme binds to the promoter and forms a closed complex E: The complex of IF-3 and the 30s small subunit of the ribosome binds to the mRNA...
1.When a protein binds to DNA at a site defined by a particular nucleotide sequence, with...
1.When a protein binds to DNA at a site defined by a particular nucleotide sequence, with which parts of the DNA does the protein primarily interact? A. the phosphate and deoxyribose groups in the DNA backbone B. the phosphate and deoxyribose groups in the DNA backbone, and hydrophobic interactions with the nucleotide bases C. nucleotide bases within the major grooves of the DNA D. nucleotide bases within the major and minor grooves of the DNA E. none of the above...
The RNA polymerase is only using one strand of DNA as a template, and only transcribes...
The RNA polymerase is only using one strand of DNA as a template, and only transcribes specific sequences. In eukaryotes, how does the RNA polymerase “know” which strand to use as a template and precisely where to initiate transcription? Explain your answer, including any relevant DNA sequences/structures and proteins needed for the process
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT