Question

Put the following statements in the order in which they occur assuming transcription happens first. A:...

Put the following statements in the order in which they occur assuming transcription happens first.

A: The initiator tRNA is located in the p-site of the ribosome

B: 50S large subunit of ribosome associates with the m-RNA-30S initiator tRNA complex

C: Initiator tRNA IF-2 GTP complex associates with pre-initiator complex

D: RNA polymerase holoenzyme binds to the promoter and forms a closed complex

E: The complex of IF-3 and the 30s small subunit of the ribosome binds to the mRNA

F: RNA polymerase holoenzyme scans the promoter

G: Sigma factor is released

H: The promoter is unwound and open complex is formed

I: IF2 and IF3 are released

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Answer #1

Following is the order of the process

  1. RNA polymerase holoenzyme scans the promoter
  2. RNA polymerase holoenzyme binds to the promoter and forms a closed complex
  3. The promoter is unwound and open complex is formed
  4. Sigma factor is released
  5. The complex of IF-3 and the 30s small subunit of the ribosome binds to the mRNA
  6. Initiator tRNA IF-2 GTP complex associates with pre-initiator complex
  7. The initiator tRNA is located in the p-site of the ribosome
  8. 50S large subunit of ribosome associates with the m-RNA-30S initiator tRNA complex
  9. IF2 and IF3 are released
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