Question

You are volunteering in a research lab and are tasked with the following question: Does myostatin treatment reduce cancer cell proliferation in vitro?

To answer this, you culture (grow) human prostate cancer cells in petri dishes and treat them with either myostatin (10 micromol/L) or pbs (as a control) for 72 hours. You measure the cell viability (% survival) as a proxy for cell proliferation. Your data are located in the table below.

1) **Now you need to analyze your data. Make sure to include your hypotheses, tests for any assumptions, whether you reject/fail to reject your null hypothesis, and your conclusion. (worth 15 points)

Cell viability, %

Myostatin	PBS
37.2	58.2
42.7	47.2
35.1	25.3
57.6	36.9
25.9	39.1
38.2	25.5
49.4	48.2
41.2	37.8
39.9	41.2
32.0	37.2
48.5	37.4
29.5	32.8
27.6	31.7
34.6	25.9
50.2	37.4
37.4	50.2
28.5	29.4
29.4	38.2
38.6	25.9

Answer 1

Solution: Here concept of t-test is used.

Conclusion: Myostatin treatment does not reduce cancer cell proliferation in vitro.