Question

When you conduct the experiment, you get unexpected results. One unexpected result is that the strain...

When you conduct the experiment, you get unexpected results. One unexpected result is that the strain containing a mutation in enzyme 4 is unable to grow in many of the conditions including with no additional compounds in the media.

b) suggest an explanation for the unexpected result.

c) Design an experiment, using one of the strains above, to test your hypothesis. Describe the results you expect to get from the experiment you design.

Engineering   Chemical-Engineering   
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Answer #1

b) D is essential OR 4 is involved in production of A, or a different essential pathway.

c)To test if D is essential: Grow 5, 3, or 1 on both A and D.(if D is essential strains would not grow on just A, butcould grow on both A and DTo test if 4 is involved in production of A: Grow strain in presence of A, and should see growth (technicallyalready did this, so just look at those results)To test if 4 is involved in a different pathway: analyze strain with mutation in 4 for accumulation ofintermediates.(what metabolites accumulate would suggest what other pathway 4 might be involved in

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