Question

How would you purify the amino acids that result from breaking down of milk protein (~ 0.01 g)

Thank you!

Answer 1

Solution:

There is no any direct purification method to purify the amino acids which break down milk into protein.

First checking with Thin Layer Chromatography (TLC) weather your compound is moving on TLC with Dichloromethane (DCM), methanol (MeOH) solvent system. If it is moving with this solvent system it's pretty easy to purify by using silica gel chromatography. If we can run it on normal silica gel Thin Layer Chromatography (TLC) plates then most likely you can do it on normal silica gel. You could also protect the carboxylic acid or amine for purification and cleave off afterwards.