Question

In paper chromatography, why should standards be run at the same time as unknowns

In paper chromatography, why should standards be run at the same time as unknowns

Engineering   Chemical-Engineering   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

The retension factor ( R​​​f ) in paper chromatography is defined as the ratio of the distance traveled by the solute to the distance traveled by the solvent. R​​​​​​f value depends on temperature and the solvent used in the experiment, so several solvents offer several R​​​​​​f values for the same mixture of compound. A solvent in chromatography is the liquid the paper is placed in and the solute is the ink which is being seperated.

The comparison between the chromatograph of the standard and the unknown gives the result. The temperature variation and the volatility of the solvent can influence the result. Therefore, inorder to eliminate the error associated with this, the paper chromatography of the standard and the unknown are carried at simultaneously using the same solvent from the same batch.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Why is it important to have filter paper in a beaker during chromatography
Why is it important to have filter paper in a beaker during chromatography
What is the principle of paper chromatography? Why can be the mixtures of dyes be separated...
What is the principle of paper chromatography? Why can be the mixtures of dyes be separated using this method?
-In gas chromatography, what is retention time? What affect would increasing the column temperature have on...
-In gas chromatography, what is retention time? What affect would increasing the column temperature have on retention time? -Why does the column length affect the ability to separate a mixture of compounds by gas chromatography? -Why is the retention time for solvents like CH2Cl2 and pentane much shorter than the compounds we dissolve in these solvents to analyze using gas chromatography? -A GC sample containing 0.5 mM cyclohexanol was analyzed by gas chromatography to give a peak for cyclohexanol in...
Pre-Lab Questions from lab Paper Chromatography of a Metal Cation Mixture 1. How are each of...
Pre-Lab Questions from lab Paper Chromatography of a Metal Cation Mixture 1. How are each of the cations, Fe3+, Ni2+ and Cu2+, visualized on the paper? 2. In the procedure below, you are specifically directed to identify each original spot using a penciled mark. Why do you suppose an ink pen cannot be used for this purpose? 3. Suppose Zn2+ where also a cation in the mixture. And further suppose the resulting chromatogram shows a spot corresponding to this cation...
1)When applying the spots to the filter paper, why is it important to keep them as...
1)When applying the spots to the filter paper, why is it important to keep them as small as possible? 2. If two dyes have the same Rf value, what might you do to resolve the identity of the two dyes using paper chromatography? 3) When developing the chromatogram, why is the beaker kept covered during the experiment? 4. What would happen if you added too much solvent and the sample spots were under the surface level of the solvent?
Should government be run like a business? why or why not?
Should government be run like a business? why or why not?
We did a lab on Gel Filtration Chromatography in which we used two "standards" Phenol Red...
We did a lab on Gel Filtration Chromatography in which we used two "standards" Phenol Red (included in gel) and Blue Dextran(excluded from gel). An unknown protein (either hemoglobin or myoglobin) was given. Question is why is a standard used (in relation to the lab) and how is it useful? Please provide a detailed answer. Thank You.
Discuss why PPP should hold in the long run. Also, why is it that PPP often...
Discuss why PPP should hold in the long run. Also, why is it that PPP often does not hold up empirically even over long periods of time.
an administrative or clinical standard report. Standard reports should ALWAYS be run with the same parameters....
an administrative or clinical standard report. Standard reports should ALWAYS be run with the same parameters. why this is the case? What is "Benchmarking" and how is it used? course: Integrated electronic health records
1. A recent article in the paper claims that government ethics are at an all-time low....
1. A recent article in the paper claims that government ethics are at an all-time low. Reporting on a recent sample, the paper claims that 41% of all constituents believe their representative possesses low ethical standards. Suppose 50 of a representativeʹs constituents are randomly and independently sampled. Assuming the paperʹs claim is correct, find the following probabilities. Find the probability that at most 20 constituents sampled believe their representative possesses low ethical standards.    Find the probability that exactly half of...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT