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In paper chromatography, why should standards be run at the same time as unknowns

In paper chromatography, why should standards be run at the same time as unknowns

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The retension factor ( R​​​f ) in paper chromatography is defined as the ratio of the distance traveled by the solute to the distance traveled by the solvent. R​​​​​​f value depends on temperature and the solvent used in the experiment, so several solvents offer several R​​​​​​f values for the same mixture of compound. A solvent in chromatography is the liquid the paper is placed in and the solute is the ink which is being seperated.

The comparison between the chromatograph of the standard and the unknown gives the result. The temperature variation and the volatility of the solvent can influence the result. Therefore, inorder to eliminate the error associated with this, the paper chromatography of the standard and the unknown are carried at simultaneously using the same solvent from the same batch.

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