Question

1)When applying the spots to the filter paper, why is it important to keep them as small as possible?

2. If two dyes have the same Rf value, what might you do to resolve the identity of the two dyes using paper chromatography?

3) When developing the chromatogram, why is the beaker kept covered during the experiment?

4. What would happen if you added too much solvent and the sample spots were under the surface level of the solvent?

Answer 1

1)When applying the spots to the filter paper, it is important to keep them as small as possible because after devellopement of the chromatogram, the spots can overlap and it will be difficult to identify.

2) If two dyes have the same Rf value, to resolve the identity of the two dyes using paper chromatography we can you either different-2 staining agent which will react and will give particular color of the dyes.

3)When developing the chromatogram, the beaker is kept covered during the experiment because the solvent which is highly volatile can evaporate.

4. if you added too much solvent and the sample spots were under the surface level of the solvent then rise of compound will be not proper as it is in side the layer of solvent and the spots can be dissolved in the solvent system