Question

How do I calculate accuracy for EIA test?

Answer 1

EIA, is a test that is used to detects and measures antibodies in our blood. This test can be used to determine if we have antibodies related to certain infectious conditions such as HIV .EIA is Enzyme-linked immunosorbent assay, also known as ELISA

A spike assay can be done to determine the accuracy of EIA results. In this assay, a known amount of recombinant protein is added or spiked or mixed in to the sample. Then by using the standard curve the amount of material is measured.

EIA data are calculated by ploting the mean absorbance against the protein concentration and draw a curve fitting to standard result , and then interpolate absorbance of samples to the curve

The value obtained after performing EIA test depends on the interaction of targeted protein with the antibodies, and this interaction is compared to a standard protein curve.

Several factors such as buffer, matrix and heterophilic antibodies may affect the binding of the sample, so care must be taken into account when evaluating the accuracy of an EIA

The above figure is standard curve . Extrapolation of absorbance to x axis is shown.In the same way we can also calculate the spike assay.