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A solution containing aspartic add, glycine, threonine, leucin

  1. A solution containing aspartic add, glycine, threonine, leucin

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Answer #1

A cation exchanger is negatively charged and hence it will hold the positively charged (basic amino acids) more.

The amino acids are eluted with an increasing ph gradient meaning first the acidic amino acids will be eluted, followed by neutral and basic amino acids will be last to elute out.

Among the given amino acids Aspertic acid is acidic

Glycin is neutral and non-polar

Threnine is neutral and polar

Leucine is neutral and non polar

Lysine is basic

Aspertic acid will elute first, leucine and glycine will elute together, then threonine , Lysine will be last to elute out

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