There are two main methods to get proteins into a gas phase for mass spectroscopy: electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Place the appropriate description of each ionization method in the appropriate space.
- Can be used for proteins or peptides that have been treated with proteases like trypsin
- Ionization evaporates solvent, leaving peptide in gas phase to travel through the separating mass spectrometer.
- Uses mass-to-charge (m/z) ratio to determine molecular mass
- Degrades and charges the peptide with high voltage
- Proteins or peptides are mixed in a solid matrix rather than a solution.
- Is based on the mass and acceleration though a chamber ending in a detector
- Laser will be absorbed by the peptide containing material releasing the peptide into the gas phase.
- Sample becomes ionized due to high voltage.
- Peptides become fragmented and charged due to laser
exposure.
Which are ESI, MALDI or BOTH?
ESI method-
-Ionization evaporates solvent, leaving peptide in gas phase to travel through the separating mass spectrometer.
- Degrades and charges the peptide with high voltage.
- Sample becomes ionized due to high voltage.
MALDI Method-
-Proteins or peptides are mixed in a solid matrix rather than a solution.
- Laser will be absorbed by the peptide containing material releasing the peptide into the gas phase.
- Peptide becomes fragmented and charged due to Laser exposure.
Both methods-
- Can be used for proteins or peptides that have been treated with proteases like trypsin.
- Uses mass to charge (m/z) ratio to determine molecular mass.
- Is based on the mass and acceleration through a chamber ending in a detector.
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