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semisolid media such as MIO are used to determine motility. Another such medium is SIM (sulfide,...

semisolid media such as MIO are used to determine motility. Another such medium is SIM (sulfide, indole, motility) medium. when would you use SIM instead of MIO?

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Answer #1

MIO is a single culture medium in which motility, indole production, and ornithine decarboxylase activity can be determined.

SIM is a signle culture medium in which motility, indole production and sulfide decarboxylase activity can be determined.

These are both differential media (media that contain various nutrients that allow the investigator to distinguish one bacterium from another by how they metabolize or change the media with a waste product) so not all bacteria is going to be able to grow on ornithine or sulfide media. If the bacteria can't be grown on MIO maybe it can be on SIM

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