Question

You are given a solution of 100 mg/ml and are asked to make solutions of 10 mg/ml, 1 mg/ml, 100 µg/ml, and 10 µg/ml. You will need 500 µl of each of the above solutions. Describe the most efficient way to end up with about 1 ml of each of the above concentrations

Answer 1

500 µl = 0.5 ml

Now, the solution available is 100 mg/ml

To make 10 mg/ml, add 9 ml of water to the solution of 100 mg/ml which will give now 100 mg/10 ml

or, the new solution will be 10 mg/ml

2) 100 µg/ml = 0.1 mg/ml

Add 999 ml of water to the solution of 100 mg/ml solution which will give 100 mg/1000 ml

or, the solution will be 0.1 mg/ml

3) 10 µg/ml = 0.01 mg/ml

Add

Add 9999 ml of water to the solution of 100 mg/ml solution which will give 100 mg/10000 ml

or, the solution will be 0.01 mg/ml