Question

What is the effect of stationary phase film thickness on gas chromatograms?

Here you must comment on retention time, peak shape, and peak resolution.

Answer 1

Stationary phase film thickness has the following effects in gas chromatograms:

a. As film thickness increases, efficiency decreases.

b. As film thickness increases, sample capacity increases.

c. As film thickness increases, retention increases which allows the separation of highly volatile solutes that have very small and close retention times.

d. The benefits of decreasing film thickness are sharper peaks (which may increase resolution) and reduced column bleed. Both of these benefits increase signal-to-noise. Additionally, the column's maximum operating temperature will be increased.