Question

In gas chromatography, what is the effect on retention time (longer, shorter, or unchanged) of the...

In gas chromatography, what is the effect on retention time (longer, shorter, or unchanged) of the following?

a. Loss of stationary phase by column bleeding.

b. Operating the column at higher temperature.

c. Operating the column at a higher head pressure.

d. Clipping off 5% of the column to remove non-volatile contaminants.

e. Changing the carrier gas from He to N2, if flow, temperature, and the column are the same.

Homework Answers

Answer #1

Here the Answer is the (b) Operating the column at higher temperature.

A excessively high column temperature results in very short retention time but also in a very poor separation because all components mainly stay in the gas phase. However, in order for the separation to occur the components need to be able to interact with the stationary phase. If the compound does not interact with the stationary phase, the retention time will decrease. At the same time, the quality of the separation deteriorates, because the differences in retention times are not as pronounced anymore. The best separations are usually observed for temperature gradients, because the differences in polarity and in boiling points are used here.

So Operating the column at higher temperature decrease the retention time, but also deteriorate the quality of the separation.

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
What effect would each of the following have on the retention times in a gas chromatography?...
What effect would each of the following have on the retention times in a gas chromatography? a) increasing the column temperature b) increasing the flow rate of the carrier gas c) increasing the amount of sample injected
-In gas chromatography, what is retention time? What affect would increasing the column temperature have on...
-In gas chromatography, what is retention time? What affect would increasing the column temperature have on retention time? -Why does the column length affect the ability to separate a mixture of compounds by gas chromatography? -Why is the retention time for solvents like CH2Cl2 and pentane much shorter than the compounds we dissolve in these solvents to analyze using gas chromatography? -A GC sample containing 0.5 mM cyclohexanol was analyzed by gas chromatography to give a peak for cyclohexanol in...
A few basic gas chromatography questions: In general, how would you determine which of several peaks...
A few basic gas chromatography questions: In general, how would you determine which of several peaks in a chromatogram obtained for a mouthwash sample might correspond to ethanol? (Is your suggested method 100% reliable?) How would you use that peak to determine how much ethanol is present in the mouthwash? In general, will an increase in operating temperature cause a given peak to have a longer or a shorter retention time? Why? In general, will a decrease in mobile phase...
In gas chromatography, (a) why do open tubular columns yield greater solute resolution than packed columns?...
In gas chromatography, (a) why do open tubular columns yield greater solute resolution than packed columns? (Check all that apply.) A.Open tubular columns have greater sample capacity. B.Open tubular columns eliminate the multiple path term (A) from the van Deemter equation. C.Open tubular columns have a lower resistance to gas flow, so longer columns can be used without increasing solute retention times. -why can more rapid flow rates be used with H2 and He carrier gases than with N2, without...
A student separated two unknown compounds using Fractional Distillation followed by Gas Chromatography. The GC column...
A student separated two unknown compounds using Fractional Distillation followed by Gas Chromatography. The GC column had a non-polar liquid stationary phase and Helium was the mobile phase. Using the following data, determine the identity, boiling point and percent composition for each of the compounds in the GC sample. Explain reasoning. Your left peak has a percent composition of 20.49% and the right has 79.6%. Hexane boiling pint is 69.2 Heptane boiling boing is 98.5 Methanol is 65.1 Octane 126...
What tools could AA leaders have used to increase their awareness of internal and external issues?...
What tools could AA leaders have used to increase their awareness of internal and external issues? ???ALASKA AIRLINES: NAVIGATING CHANGE In the autumn of 2007, Alaska Airlines executives adjourned at the end of a long and stressful day in the midst of a multi-day strategic planning session. Most headed outside to relax, unwind and enjoy a bonfire on the shore of Semiahmoo Spit, outside the meeting venue in Blaine, a seaport town in northwest Washington state. Meanwhile, several members of...
Delta airlines case study Global strategy. Describe the current global strategy and provide evidence about how...
Delta airlines case study Global strategy. Describe the current global strategy and provide evidence about how the firms resources incompetencies support the given pressures regarding costs and local responsiveness. Describe entry modes have they usually used, and whether they are appropriate for the given strategy. Any key issues in their global strategy? casestudy: Atlanta, June 17, 2014. Sea of Delta employees and their families swarmed between food trucks, amusement park booths, and entertainment venues that were scattered throughout what would...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT