What does an absorbance vs eluent graph tell you?
why is blue dextran analyized at 650nm?
why is myoglobin analyzed at 500nm?
why is potassium chromate analyzed at 440nm?
What does an absorbance vs eluent graph tell you?
The separation of the components of a mixture based on different attractive forces that they have with the adsorbent (fixed phase). The more strongly attracted by the adsorbent will be the more difficult to elute with the solvent (mobile phase) and will be the least moves. On this basis the various pigments are separated according to their affinity for the stationary phase as well as the eluent.
why is blue dextran analyized at 650nm?
As a result of the interactions between photons and the absorbent particles of the solution transmittance is the fraction of radiation transmitted (or absorbed) by the solution incident. Photometer using a red filter, and wavelength of 650nm the solution is blue-green.
why is myoglobin analyzed at 500nm?
Myoglobin absorb green to yellow light in the 500 nm to 600 nm region of the spectrum. Red light is
transmitted through solutions containing these proteins so the solutions appear to be red in color!
why is potassium chromate analyzed at 440nm?
it has an absorption maximum at 440 nm
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