Question

What does the slope of a calibration line represent? (This line is for absorbance vs. concentration, and has a slope of 2.301 with a y-intercept of .0069.)

Answer 1

The slope of a line can be determined using the formula y2-y1/x2-x1.

According to Beer-Lambert's law, the absorbance is directly proportional to concentration of the solution. A=€Cl

Generally the equation for the calibration curve is

Y= m x

Y--- absorbance

X-- concentration

m -- slope, will be calculated from graph.

If we know the any of the value( either abs or concn, we can find the other using slope)

For some curves has the equation Y = MX + C

In the similar fashion, the values can be determined..

The slope at any point on the line should be constant. Otherwise it is not a linear curve.