Question

What does the slope of a calibration line represent? (This line is for absorbance vs. concentration, and has a slope of 2.301 with a y-intercept of .0069.)

Answer #1

The slope of a line can be determined using the formula y2-y1/x2-x1.

According to Beer-Lambert's law, the absorbance is directly proportional to concentration of the solution. A=€Cl

Generally the equation for the calibration curve is

Y= m x

Y--- absorbance

X-- concentration

m -- slope, will be calculated from graph.

If we know the any of the value( either abs or concn, we can find the other using slope)

For some curves has the equation Y = MX + C

In the similar fashion, the values can be determined..

The slope at any point on the line should be constant. Otherwise it is not a linear curve.

if the slope of the calibration curve of absorbance vs
concentration is 0.0681x+0 (mg/mL) how would I determine the
concentration of a diluted solution (1/8 diluted with 0.424
absorbance)

What does the slope and intercept of a Force vs Velocity Squared
graph represent? And how do you calculate the theoretical values
for the slope and intercept?

In a graph of v1 vs v2. What does the slope represent?
For a perfectly inelastic collision.
They have the same mass.
x-axis is v1 (velocity 1) and y-axis is v2 (velocity 2).
What does the slope represent? And how do we measure this
quantity using mathemtacial formula?
(From physical lab experiment)

Using Excel and least-square line (aka linear regression)
fitting, find the slope, y-intercept, and correlation coefficient
(R2) for the calibration of Absorbance (A) vs.
Concentration (C in M) of Vitamin C at 246 nm UV light as outlined
for Beer’s Law: {C, A} = {{1.414, 81.10}, {1.359, 84.38}, {0.637,
28.30}, {1.829, 86.46}, {1.520, 94.40}, {0.260, 29.33}, {1.186,
44.50}, {1.509, 70.76}}. Formally plot the points with the
superimposed fitted line with the proper title, axis labels and
units, etc. What is...

The calibration plot (Absorbance of Cu+2 vs Cu+2 concentration
(mol/L)) is: A= 4.685x +0.0363
Consider CuCl2. xH2O. The mass of CuCl2. xH2O used to prepare
the solution is 0.195 g and the mass of the solution is 8.987 g.
The density of the solution is determined to be 0.998 g/mL.
a.) calculate the volume of the solution in L.
b.) If the solution has an absorbance of 0.632, calculate the
concentration of Cu+2 in the prepared solution
c.) calculate the...

The data in the table at the right show a calibration curve for
the determination of RNA from its absorbance at 260 nm. Use a
spreadsheet and the least-squares method to determine the slope and
intercept of the best straight line fit to the calibration curve.
(Do not subtract the blank reading when creating your calibration
curve.)
RNA /absorbance at 260nm
0.00 /.243
10.09/.460
20.18/.788
30.27/1.072
40.36/1.303
find slope in abs/ug and intercept
Using the slope and intercept of the...

A calibration curve for CuSO4 at 635 nm yields a straight line
described by the equation y = 2.182 x + 0.968. If the measured
absorbance for a solution of unknown concentration of CuSO4 is
0.226, what is the concentration of the CuSO4 solution? Provide
your response to three digits after the decimal. _____ M

1. What does a positive and negative slope represent for a
position versus time graph?
2. What does a negative slope represent for a position versus
time graph?
3. What does a straight line (0 slope) represent for a position
versus time graph?
4. What does a positive slope represent for a velocity versus
time graph?
5. What does a negative slope represent for a velocity versus
time graph?
6. What does a straight line (0 slope) represent for a...

Suppose an unknown sample has an absorbance which is significantly
larger than the absorbance of the most concentrated standard and so
is outside the range of the calibration curve. What should the
analyst do to accurately determine the concentration for the
unknown using Beers law? Extending the calibration curve or using
the equation for the line are not correct answers to this
question.

Calibration data for a bromide ion-selective electrode (ISE) was
collected and recorded below. The potential of the ISE was measured
against a saturated calomel electrode (SCE). All solutions were
buffered at a pH of 7.56. A linear calibration curve can be
constructed from this data as a plot of potential (in mV) vs. pBr.
Determine the slope and y-intercept of such a plot of the
calibration data given.
[Br-](M) Potential (mV)
0.300 -21.2
0.0300 36.7
0.00300 96.0
0.000300 154.7
0.0000300...

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