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Clearly outline the steps you would take to determine the detection limit for an absorbance measurement...

Clearly outline the steps you would take to determine the detection limit for an absorbance measurement based around Beer's Law. Identify the data that you would need to collect and how you would use the data to calculate LOD.

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Answer #1

The detection limit is the concentration that corresponds to a signal that is three (or two) times
the standard deviation of the background above the background. In order to determine the
detection limit, you need multiple measurements of the blank to define the background and its
standard deviation. With these values, you can calculate the signal at the detection limit as
SIgnal (dl) = Signal(blank)+ 3s(blank). You must then convert this signal into a concentration. This can
be done by preparing a calibration curve by measuring the absorbance at several known
concentrations and using the Beer’s law relationship to convert the SignalDL (which is an
absorbance) into its corresponding concentration.

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