Question

Clearly outline the steps you would take to determine the detection limit for an absorbance measurement...

Clearly outline the steps you would take to determine the detection limit for an absorbance measurement based around Beer's Law. Identify the data that you would need to collect and how you would use the data to calculate LOD.

Science   Chemistry   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

The detection limit is the concentration that corresponds to a signal that is three (or two) times
the standard deviation of the background above the background. In order to determine the
detection limit, you need multiple measurements of the blank to define the background and its
standard deviation. With these values, you can calculate the signal at the detection limit as
SIgnal (dl) = Signal(blank)+ 3s(blank). You must then convert this signal into a concentration. This can
be done by preparing a calibration curve by measuring the absorbance at several known
concentrations and using the Beer’s law relationship to convert the SignalDL (which is an
absorbance) into its corresponding concentration.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Outline some specific steps an organization might take to perform data cleansing to ensure the accuracy...
Outline some specific steps an organization might take to perform data cleansing to ensure the accuracy and completeness of its customer database before adding this data to a data warehouse. How would you decide when the data is accurate enough?
Outline some specific steps an organization might take to perform data cleansing to ensure the accuracy...
Outline some specific steps an organization might take to perform data cleansing to ensure the accuracy and completeness of its customer database before adding this data to a data warehouse. How would you decide when the data is accurate enough?
II. I want you to make up a research project that you would like to do....
II. I want you to make up a research project that you would like to do. You will be guided through a step by step process of giving me all of the necessary information for a research plan. Be careful to answer each question completely using what you have learned about research design. You have decided to do a research project. A. Describe your project. What is your purpose, what question or questions are you trying to answer. B. What...
For the scenarios below, please say what evaluation approach you would use (Randomization, IV, RD, Diff-in-diff)...
For the scenarios below, please say what evaluation approach you would use (Randomization, IV, RD, Diff-in-diff) and provide details on how you would set up your evaluation (data needed and regression model, specifying dependent variables and explanatory variables). Q. The lost boys of Neverland build a few artificial reefs around several of their coastal villages. Artificial reefs are great for marine life and are believed to stimulate tourism. Yet, as any good economist, the lost boys don’t want to take...
1) Use Henry's law to determine the molar solubility of helium at a pressure of 1.9...
1) Use Henry's law to determine the molar solubility of helium at a pressure of 1.9 atm and 25 ∘C. Henry’s law constant for helium gas in water at 25 ∘C is 3.70⋅10−4M/atm. 2) A 2.800×10−2M solution of NaCl in water is at 20.0∘C. The sample was created by dissolving a sample of NaCl in water and then bringing the volume up to 1.000 L. It was determined that the volume of water needed to do this was 999.2 mL...
**( Please show steps on excel and formulas used )** In this project, you’ll use the...
**( Please show steps on excel and formulas used )** In this project, you’ll use the web to research alternative wireless services and use spreadsheet software to calculate wireless service costs for a sales force. You would like to equip your sales force of 35, based in St. Louis, Missouri, with mobile phones that have capabilities for voice transmission, text messaging, Internet access, and taking and sending photos. Use the web to select two wireless providers that offer nationwide voice...
Create a hypothetical scenario where you need to use a one-way ANOVA to test your hypothesis....
Create a hypothetical scenario where you need to use a one-way ANOVA to test your hypothesis. In a Word document or PDF answer the questions below. Label your submission clearly so it's easy to figure out which sections you're answering. 1. In a couple sentences, describe the study design and how the independent and dependent variables were measured (mentioning the scale of measurement: nominal, ordinal, interval, ratio). 2. In SPSS, create a set of data that has at least 10...
You are considering the purchase one Telstra Ltd share on 1 July 2017. You will purchase...
You are considering the purchase one Telstra Ltd share on 1 July 2017. You will purchase the share on the ASX. a)      Use the Yahoo7 Finance website to find the beta for Telstra. b)      You will need a proxy for return on the market. To get this, calculate the return on the ASX 200 index for the year ended 30 June 2017. Use the Yahoo7 Finance website to collect the ASX 200 data. Show your workings. c)      You will need...
This week you will collect time measurements and organize your observed data into a frequency distribution...
This week you will collect time measurements and organize your observed data into a frequency distribution table and a relative frequency histogram. Start by choosing something you can time easily in seconds. (Thing about events that typically take between 20 seconds and 3 minutes.) You will need to collect at least 30 observations. Be as consistent as you can with your time measurements. Most of you have smart phones with easy-to-use apps with timers already built in. If your measurements...
To cope with the current factory closures GM would like to replace an old parts producing...
To cope with the current factory closures GM would like to replace an old parts producing machine with a 3d printer. The old machine was bought 10 years ago and at that point was expected last for 20 years. Initially $1,000 was spent bringing and installing it. The new printer would cost $100,000. The old machine cost $40,000 and had been expected to have a salvage value of $0 (zero) in twenty years. The new printer is expected to last...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT