Question

After peptide bond formation: Group of answer choices A. Both AA are connected to the A...

After peptide bond formation:

Group of answer choices

A. Both AA are connected to the A site tRNA

B. Both AA are connected to the P site tRNA

C. Both AA are connected to the E site tRNA

D. One AA is connected to the A site tRNA and one to the P site tRNA

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Answer #1

ANSWER A. Both the amino acids are connected to the A site tRNA

The large ribosomal subunit has 3 sites in the given order- E, P, and A. The initiation complex is formed at the middle P site and consists of mRNA, initiator tRNA (tRNA 1),the large and small units of the ribosome. For elongation of chain i.e. addition of next amino acid, the next charged tRNA (tRNA 2) lands at A site (where next codon on mRNA is revealed). The peptide bond is formed and the amino acid on tRNA1 also shifts to the tRNA 2 (present on A site). The mRNA shifts/moves by one codon thus shifting tRNA 1 to E site from where it exits the complex and tRNA 2 to the P site. This tRNA 2 is carrying both the amino acids bound through a peptide bond. The cycle repeats similarly for further addition of amino acids.

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