Question

25.       The composition of YT media is 2.0% NaCl, 1.0% peptone and 0.5% yeast extract. How much of each of these components do you need to weigh out in order to prepare 500 ml of media?

Answer 1

2.0 NaCl means 2 g of NaCl should be added to 100 mL of media. So, for 500 mL of the media, 2*5 = 10 g of NaCl should be added.

1.0% peptone means 1 g of peptone should be added to 100 mL of media. So, for 500 mL of the media, 1*5 = 5 g of peptone should be added.

0.5% yeast extract means 0.5 g of yeast extract should be added to 100 mL of media. So, for 500 mL of the media, 0.5*5 = 2.5 g of yeast extract should be added.

So, inorder to prepare 500 mL of YT media, you need to weigh out

NaCl - 10 g

Peptone - 5 g

Yeast extract - 2.5 g