Question

To extract DNA from a tissue sample, you need to prepare 500 ml extraction buffer. You have thefollowing components, along with water:

Stocks

3 M NaCl

10% (w/v) sodium dodecyl sulfate

20 mg/ml Proteinase K

You need your extraction buffer to contain:

Final

30 mM NaCl

0.2% (w/v) sodium dodecyl sulfate

1.5 mg/ml Proteinase K

How much of each is needed to make your extraction buffer? How much water is needed?

Amount needed:         Component:

____________            3 M NaCl

____________            10% (w/v) sodium dodecyl sulfate

____________            20 mg/ml Proteinase K

____________           water

Answer 1

Amount needed	Component
87.67 gm	3 M Nacl
50 gm	10% (w/v) sodium dodecyl sulfate
10 gm	20mg/ml proteinase K
500 ml	Water