Question

Give a brief description of fructose 1,6 bisphosphate aldolase. In what pathway does the enzyme occur? What reaction does the enzyme catalyze? What type of reaction is catalyzed? What are the intermediates in the reaction? What are the cofactors? What mechanisms are employed to achieve catalysis (acid-base, covalent, nucleophilic, etc???) What type of regulation controls the activity of the enzyme?

Answer 1

Answere : Fructose-1,6-bisphosphate aldolase is a heterotetrameric aldolase enzyme that catalyze reversible cleavage of fructose-1,6-bisphosphate into dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GAP).

This enzyme involves in glycolysis and gluconeogenesis. The enzyme perform aldol reaction (i.e. making or degrading aldol).

Glycolysis is the breakdown of glucose into two pyruvic acids molecule along with generation of two ATP molecules i.e.

Glucose + 2 NAD +2 ADP + 2 Pi = 2 pyruvate + 2 ATP + 2 NADH + 2 H2O + 4 H+

Gluconeogenesis is the formation of glucose from non-carbohydrate source.

There is not much known about regulation of this aldolase. But it may be regulated at transcription and translation level by regulating the mRNA synthesis. cAMP play a role in its expression and effect its gene expression.