Question

For this assignment, you will need to access GenBank by going to the NCBI website and using the dropdown menu to search “Nucleotide.” Note that the definition of the coding strand is the strand of DNA within the gene that is identical to the transcript. On the other hand, the template strand is the strand that is complementary to the coding strand. a. Use the following accession number to access the nucleotide sequence in GenBank: NM_001018168 b. Go to the FEATURES section of the record. c. Name the protein product of the CDS. d. Write the first four amino acids (starting from the N-terminus). e. Write the nucleotide sequence of the coding strand that corresponds to these amino acids. f. Write the nucleotide sequence of the template strand that corresponds to these amino acids. g. Using the sequence shown in the record, give the nucleotide number range that corresponds to these amino acids.

Answer 1

C) The protein product of the CDS is RecQ type DNA helicase.

d) The first four amino acids starting from N-terminus are methionine, valine,valine and alanine (N-Met-val-val-ala-C).

e) Nucleotide sequence of the coding strand that corresponds to these amino acids are

5'-ATGGTCGTCGCT-3'.

f) Nucleotide sequence of template strand that corresponds to these amino acids are

3'-TACCAGCAGCGA-5'.

g) The nucleotide number range that corresponds to these aminoacids is 1-12.