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How to understand which type of restriction enzymes to use in an experiment and what will...

How to understand which type of restriction enzymes to use in an experiment and what will happen if they were damaged and did not work?
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Answer #1

The restriction enzyme has specific site at which they will cut the DNA. These sites are palindromic in nature. Palindrome is the site which has same sequence from left and right side. For example: MALAYALAM.

We should first look in the DNA sequence, then find these palindromic site and look for the particular restriction site for a specific enzyme. Now the appropriate enzyme is used to cut the DNA. This leads to cut the DNA into fragments.

If the enzyme is damaged or it did bot work, then the enzyme can not find the palindromic and its appropriate site of binding on the DNA. That's why there is not found digestion of DNA. There is not found any cut in the DNA. Hence the fragments remains in uncut form.

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