Question

1. In the last experiment, you set up the experiment with M. tuberculosis, but again replacing the air in the chamber with nitrogen gas as you did in the second experiment. You get the following results, which you do not need to graph.

Time (minutes)	no sugar	glucose	sucrose	maltose	lactose	galactose
0	0	0	0	0	0	0
5	0	0	0	0	0	0
10	0	0	0	0	0	0
15	0	0	0	0	0	0
20	0	0	0	0	0	0
25	0	0	0	0	0	0
30	0	0	0	0	0	0
35	0	0	0	0	0	0
40	0	0	0	0	0	0
45	0	0	0	0	0	0
50	0	0	0	0	0	0
55	0	0	0	0	0	0
60	0	0	0	0	0	0

a) There was clearly no CO2 produced in any of the eudiometers. Nothing. Explain in your own words why some sugars were consumed with air in the flask, but not with N₂.

Answer 1

There was clearly no CO2 produced in any of the eudiometers.for this

•Let us see characters about Mycobacterium tuberculosis

-It is a slow-growing obligate aerobe and a facultative intracellular parasite.

-TB is caused by Mycobacterium tuberculosis.

-An obligate aerobe is an organism that requires oxygen to grow. Through cellular respiration, these organisms use oxygen to metabolise substances, like sugars or fats, to obtain energy. In this type of respiration, oxygen serves as the terminal electron acceptor for the electron transport chain.

-If there is total absence of Oxygen , there is low survival chance of bacterium in that medium . And in the absence of Oxygen there is no breakdown of any sugar [carbohydrate molecule] and so CO2 is not released.