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Determine the number of dilution steps for five 20-fold serial dilutions?

Determine the number of dilution steps for five 20-fold serial dilutions?

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Answer #1
  • from the stock solution we take 1 ml and add it to 19 ml of water so as to dilute it . then the total volume of resulting solution is 20 ml
  • Dilution factor (DF)  = total volume / sample volume = 20 / 1 = 20
  • that is 20 fold dilution , which means that it is diluted to 1/20
  • if w do 5 such dilution , then
  • dilution = 1/20 x 1/20 x 1/20 x 1/20 x 1/20 = 1/3200000
  • dilution factor = 3.2 x 10^6
  • so it is 3.2 x 10^6 fold dilution
  • number of dilution steps = 5
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