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The donor and the recipient cells were combined in a microcentrifuge tube before plating them onto...

The donor and the recipient cells were combined in a microcentrifuge tube before plating them onto the "conjugation" plate. If nucleases were already present in the tube before proceeding as above, would conjugation still take place? Why or why not?

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Answer #1

Conjugation is a process in which bacteria transfer their genetic material through the cell to cell contact. During this process, the donor cell produces a pilus or a bridge like connection which aids in the transfer of genetic material from the donor to the recipient.

In the given question, the donor and recipient cells were mixed in a tube which has nuclease. This should not affect the conjugation. Nucleases do not iterfere with the conjugation as it take place through the pilus. Plli are generally resistant to nuclease treatment and hence, the transfer of genetic material from the donor to the recepient take place smoothly.

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