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why is soft agar used in the plaque assay

why is soft agar used in the plaque assay
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Plaque assay can be used to purify a clonal population of virus. A viral plaque is a visible structure formed within a cell structure such as bacterial cultures within some nutrient medium for example agar. The bacteriophage virus replicate and spread and generates regions of cell destructions known as plaques. The medium used in phage plaque assays has a relatively low percent of agar and therefore it is known as soft agar. It permits diffusion of phage to nearby uninfected cells but not permit new phages to move to remote parts of the plate.

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