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Compare the Bradford assay and the Bicinchonic Acid Assay. Which is likely to give the most...

Compare the Bradford assay and the Bicinchonic Acid Assay. Which is likely to give the most accurate estimate of protein concentration.

Explain why A280can be used to measure protein concentration. Is this method better suited to complex samples or purified proteins?

After preparing a standard curve of diluted BSA protein in a BCA assay you get a trendline with the equation A562 = 0.0011[BSA] (mg/mL) + 0.0378.

Rearrange the equation to solve for [BSA] (mg/mL).

If a 1:8 dilution of sample gives a A562 reading of 0.324, what is the concentration of protein in the undiluted sample?

Given a total volume of 5.5mL, what is the total amount of protein in the sample?

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