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How would you expect the lanes on a gel to differ if you cut the same...

How would you expect the lanes on a gel to differ if you cut the same DNA with each type of enzyme and why? (3)
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Answer #1

If we cut the DNA with different types of type 2 restriction enzymes then the corresponding bands on the various lanes will differ according to there molecular sizes and also in the total number of bands present on lane. The main reason for this observation is that different restriction enzyme will cut according to their recognition sites which differ from enzyme to enzyme and also different number of bands will be due to the frequency of the recognition sites that come on DNA of that particular emzyme.

Also the different recognition enzymes like type 1 type 2 and type 3 etc will differ on there gel profile based on how near they cut the DNA to the site they recognise i.e. whether they cut same DNA sequence which they recognise.

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