Question

2) Answer the following questions:

What is the size of the lambda DNA?

What is the recognition sequence recognized by HindIII?

How many times will the enzyme cut the lambda DNA?

What is the driving force that propels the DNA though the gel?

Where should the positive electrode be positioned related to the gel?

Using the gel in the virtual lab. If the lanes are numbered 1-4 (from left to right), which lane contains uncut DNA? How do you know this DNA is uncut?

Answer 1

1. Linear dsDNA, 48502 bp

2. 5' A AGCTT 3'

3' TTCGA A 5'

(gap represents cut site)

3. 8 fragments, 7 cut sites

4. The agarose gel acts as a molecular sieve for the separation of fragment of DNA. The separation takes place on the basis of size. Smaller the size of the fragment, faster it moves on the gel and appears farthest from the loading well.

5. The positive electrode is positioned away from the loading well and the negative is at the loading well.

Please rate high.