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why would you want your restriction enzyme to cut as close as possible to the insulin...

why would you want your restriction enzyme to cut as close as possible to the insulin gene without cutting into it?

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Q. Ans:All of this has to do with DNA conservation. If you cut the insulin gene just once, and as similar as possible to the insulin gene, you can keep the insulin gene out and very little else. Each cut has the potential to lose DNA because of wastage.

To make sure that the desired information is transferred to the plasmid without adding extra unknown or undesirable sequences. These are the reasons why restriction enzyme cut as close as possible to insulin gene without cutting onto it.

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