Question

I prepared a serial dilution series by adding 50 µl of the original undiluted sample to 4.95 ml of sterile diluent. I then repeated this step once using the diluted sample (rather than the original sample). I then added 0.5 ml of the doubly diluted sample to 4.5 ml of sterile diluent

What is the total dilution of the original sample?

Answer 1

At the last step total dilution of the original sample is 10^-5 dilution or 1:100000 dilution.

Details step are given in the following image