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Which of the following method uses “Heat Shock”?

Which of the following method uses “Heat Shock”?

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Ans- heat shock method is used for bacterial transformation. transformation means the ingestion of foreign DNA or plasmid DNA from its surroundings. in the heat shock method calcium-rich environment used to counteract the electrostatic repulsion between DNA and bacterial membrane (competent bacterial cell). in heat shock method cells are placed into water bath at 42 *C for 30 seconds.  the sudden increase in temperature crates pore in the bacterial membrane which allows the enters of the DNA into the competent bacterial cell. after heat shock cells are immediately cooled in ice.

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