Question

Although the Cleavage polyadenylation specificity factor (CPSF) is responsible for cleavage of the RNA, cleavage doesn't occur until after recruitment of CF1, CF2 and polyA polymerase. Suppose the auxin inducible degron was used to remove CF1. When compared to the termination defects due to loss of other components of the polyadenylation complex, loss of CT1 would resemble loss of which other factor?

a-PP1

b-CPSF73

c-XRN2 and PP1

d-XRN2

Answer 1

Answer: D (XRN2)

CPSF73-dependent changes occurring termination beyond the PAS by direct contribution. In the absence of other factors with the ability for XRN2 promotes termination. Depletion of XRN2 causes termination defects on only a subset of genes. Although human PCF11 is recruited to genes irrespective of XRN2 some PAS-dependent processes allows XRN2 function. Therefore, XRN2 pauses Pol-II downstream from a PAS, thus, pausing enhances termination.

Rapid elimination of XRN2, via an Auxin-inducible degron, occurs general termination defect on protein-coding genes loss of XRN2.

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