Question

“DNA Type”	“Longest Length (in base pairs)”
“Foreign”	720
“Plasmid”	2804

“Post-Lab Questions”

“1. What is the expected size of the plasmid plus the cut foreign DNA?”

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“2. What type of ends to the enzymes BamHI and EcoRI produce? How does this type of end facilitate cloning?”

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“3. What enzyme is necessary to permanently link the digested foreign and plasmid DNA together to form the recombinant DNA molecule? How does this enzyme work?”

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“4. How would you clone a gene into a plasmid if there were no common restriction sites between the two DNA sequences?”

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Answer 1

1.

* Expected size of the plasmid(2804) plus the cut DNA (720) IS 3524 bp.

Answer for 2.

* The end of the enzymes BamHI and EcoRI produce sticky ends.The sticky ends of BamHIand EcoRI help faciliate cloning by having a high probability of attracting each other in a solution.

Answer for 3

The enzyme that is necessary to permanently link the digested foreign and plasmid DNA together to form the recombinant DNA is a DNA ligase.Which is the catalyst needed to create a bond between RNA And DNA .

Answer for 4

Cloning a gene into a plasmid.if there were no common restriction sites between the two DNA sequence would involve identify and select a unique site in the plasmid by cutting a fragment that is not necessary and replace it with the need fragment using a ligand.