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Assuming that each of the 5 squares usually counted on the hemocytometer contains the exact same...

Assuming that each of the 5 squares usually counted on the hemocytometer contains the exact same number of cells counted in the single square shown, determine the stock culture concentration in exponential form. The stock culture was then diluted to a final concentration of 1000 cells/mL.

a) Calculate the stock culture concentration and the total dilution factor. Show your formula and equations.

b) Illustrate how to dilute the stock culture to the final concentration in Word. Include in your illustration the volume of saline in each tube, the volume of culture measured from the previous tube, the dilution at each step, and the total dilution factor. Upload your word file.
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Answer #1

Dilution = initial conc / final conc = 380000 / 1000 = 380

Dilution factor = 1 / dilution = 1 / 380

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