Question

The Hofmeiser series given as citrate>phosphate>sulphate>acetate>chloride>nitrate. From these series select the correct response to the following statements: nitrate salts are more effective than phosphate salts for salting out—true/false?

Nitrate salts tend to cause more structural damage to proteins than phosphate salts—true/false.

Answer 1

Solution:

FALSE

Salts with high values of KS are more effective for salting-out than salts with low KS. The Hofmeister series gives the relative effectiveness of different anions for protein precipitation And Nitrate salts have low value of Ks.

Answer 2

TRUE

The salts at the low end of this series tend to cause structural damage to proteins. As nitrate is at lower end so do more structural damage to proteins than phosphate salts.

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