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Firefly luciferase is the enzyme that allows fireflies to illuminate their abdomens. Because this light generation...

Firefly luciferase is the enzyme that allows fireflies to illuminate their abdomens. Because this light generation is an ATP-requiring reaction, firefly luciferase can be used to test for the presence of ATP. In this way, luciferase can test for the presence of life. The coupled reactions are

luciferin+O2ATP??oxyluciferin+lightAMP+PPi

If the overall ?G of the coupled reaction is -7.10kJ/mol , what is the equilibrium constant, K, of the first reactions at 18?C ? The ?G for the hydrolysis of ATP to AMP is ?31.6 kJ/mol.

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