Firefly luciferase is the enzyme that allows fireflies to illuminate their abdomens. Because this light generation is an ATP-requiring reaction, firefly luciferase can be used to test for the presence of ATP. In this way, luciferase can test for the presence of life. The coupled reactions are 1.2.luciferin+O2ATP⇌⇌oxyluciferin+lightAMP+PPiΔG∘=−31.6 kJ/mol If the overall ΔG∘ of the coupled reaction is -5.80 kJ/mol , what is the equilibrium constant, K, of the first reaction at 28 ∘C ?
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