Question

Kenny needs to make a 2 L of 10 mM Tris Buffer pH 8.3. He has available, solid TrisHCl, Solid Tris Base, 10 N sodium hydroxide, and Conc HCl. Write out (a) calculations & (b) protocol/procedure.

Must answer both parts of the question in full to get any points (No spam or fluff)!

Answer 1

Preparation of Tris buffer for pH 8.3.

The important chemicals for preparation are: Tris powder, distilled water,hydrochloric acid, pH meter, and glassware

Prepareat 2 L of 10 mM Tris Buffer: The following procedure is use in preparatio of tris buffer:

1. moles of Tris = mol/L x L

Moles of tris= 10x2= 20

2. grams of Tris = (moles) x (121.14 g/mol) -- molecular weight of Tris(121.14 g/mol

grams of tris = 20x121.14 g/mol = 2422.8 g/mol.

3. Dissolve the Tris into distilled deionized water to get the volume to 2L.