Question

Imagine that in a metabolic pathway, one of the steps involves the oxidation of saturated compound to an unsaturated compound, with the concomitant reduction of the cofactor FAD to FADH2 as shown below:

R-CH2-CH2-R' + FAD <----> R-CH=CH-R' + FADH2

Imagine further that ΔG°´ for this reaction is 0 kcal/mol. Given this scenario, what would be the equilibrium concentration of FADH2 (expressed in mM to the nearest tenth of a unit) if a biochemist started with a solution that contained 0.009 M each of R-CH2-CH2-R', FAD, and FADH2 (but no R-CH=CH-R'), along with 24 nM concentration of the enzyme that catalyzes the reaction?

Answer 1