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Why is it important to force the regression line in the Beer’s Law plot of absorbance...

Why is it important to force the regression line in the Beer’s Law plot of absorbance vs. concentration to go through the origin? Would adding the point (0,0) to your data set have had the same affect? Why or Why not? (Beers law Lab)

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Answer #1

Why is it important to force the regression line in the Beer’s Law plot of absorbance vs. concentration to go through the origin?

The importance of the linear regression through the origin , is that a correction of absorbance reading is made, since not read a sample and read only blank sample absorbance should be zero.

Would adding the point (0,0) to your data set have had the same affect? Why or Why not?

Add the point (0,0) have a very different effect , not added is assumed that the line begins in an absorbance value ( bo) , not the relationship be fulfilled.

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