Question

Determine the PI of peptide AEGK (Ala-Glu-Gly-Lys).

Determine the PI of peptide AEGK (Ala-Glu-Gly-Lys).

Homework Answers

Answer #1

PI is the average of the pKa values representing the protonation and deprotonation of the neutral form of the peptide.

The pH range where the peptide has a net charge of 0 is between pH 4.2 and pH 9.7.

Below pH 4.2, which represents the pKa value for the glutamic acid carboxyl group, the peptide gains a net charge of +1.

Above pH 9.7, which represents the pKa value for peptide N-terminus, the peptide has a net charge of -1.

Therefor, PI = (4.2+9.7)/2 = 6.95

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Can you calculate the pI for the following peptide? Phe-Lys-Glu-Asp-Lys-Ser-Ala (note: there is one alpha carboxyl...
Can you calculate the pI for the following peptide? Phe-Lys-Glu-Asp-Lys-Ser-Ala (note: there is one alpha carboxyl at the C-terminus and one alpha-amino group at the N- terminus and don't forget to take into account the ionizable side chain groups!)
Calculate the net charge on this polypeptide: Glu-Gly-Ala-Lys. What is the charge at a pH of...
Calculate the net charge on this polypeptide: Glu-Gly-Ala-Lys. What is the charge at a pH of 1? Please show work, I'm so confused!
Which of the following polypeptides is most likely to form an alpha helix? Explain? a) Cys-Arg-Ala-Gly-Asn-Arg-Lys-Ile-Leu-Glu-Thr-Tyr...
Which of the following polypeptides is most likely to form an alpha helix? Explain? a) Cys-Arg-Ala-Gly-Asn-Arg-Lys-Ile-Leu-Glu-Thr-Tyr b) Ser-Glu-Asp-Asn-Phe-Gly-Ala-Pro-Lys-Ser-Ille-Leu-Trp c) Gln-Lys-Ala-Ser-Val-Glu-Met-Ala-Val-Arg-Asn-Ser-Gly
For the peptide Ala-Gly-Lys-Tyr-Ser-His, the number of free amino (NH2) groups is
For the peptide Ala-Gly-Lys-Tyr-Ser-His, the number of free amino (NH2) groups is
Predict the charge on the major species of the peptide Ile–Lys–Glu–Ala–Arg–Ser–Asn at pH 7.0
Predict the charge on the major species of the peptide Ile–Lys–Glu–Ala–Arg–Ser–Asn at pH 7.0
Consider the following short peptide: Ala-Gly-Lys-Ser-Asp What is the difference in the number of primary amines...
Consider the following short peptide: Ala-Gly-Lys-Ser-Asp What is the difference in the number of primary amines this peptide would yield after it was fully hydrolyzed versus when it was intact as shown (report the absolute/positive value)?
Predict the charge on the major species of the peptide Gly-Arg-Asp-Ala-Lys-Ser-Asn at PH 6.0. Please, can...
Predict the charge on the major species of the peptide Gly-Arg-Asp-Ala-Lys-Ser-Asn at PH 6.0. Please, can you explain? Thanks.
For the peptide Ala-His-Glu-Val-Asp-Cys-Lys-Leu what is the net charge at pH 3? Please explain how you...
For the peptide Ala-His-Glu-Val-Asp-Cys-Lys-Leu what is the net charge at pH 3? Please explain how you get the charge of each amino acid.
This peptide, Lys-Glu-Ala-Asp-Arg-Met-Arg, will be isoelectric at what pH range? A. neutral b. acidic c. basic...
This peptide, Lys-Glu-Ala-Asp-Arg-Met-Arg, will be isoelectric at what pH range? A. neutral b. acidic c. basic d. very basic The answer is C. Can you please explain how to figure this out.
Write down the mRNA sequence for: met-ser-leu-gly-trp-stop met-ser-pro-cys-his-stop met-ile-asn-ala-tyr-stop met-gln-thr-leu-arg-cys-pro-lys-stop start-ile-glu-asp-ser-his-trp-stop start-leu-pro-gln-tyr-ile-met-val-ala-stop start-ala-glu-trp-ser-phe-pro-
Write down the mRNA sequence for: met-ser-leu-gly-trp-stop met-ser-pro-cys-his-stop met-ile-asn-ala-tyr-stop met-gln-thr-leu-arg-cys-pro-lys-stop start-ile-glu-asp-ser-his-trp-stop start-leu-pro-gln-tyr-ile-met-val-ala-stop start-ala-glu-trp-ser-phe-pro-ser-gly-thr-stop start-val-ala-thr-thr-leu-tyr-cys-gly-arg-stop start-lys-asn-gly-phe-his-thr-arg-pro-gln-stop start-met-thr-asn-lys-pro-gln-ser-leu-arg-stop
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT